Tabrizicola piscis sp. Nov., isolated from the intestinal tract of a korean indigenous freshwater fish, acheilognathus koreensis

A novel Gram-negative, obligately aerobic, rod-shaped and non-motile bacterium, designated strain K13M18^T, was isolated from the intestinal tract of a Korean indigenous fish, oily bitterling (Acheilognathus koreensis). Strain K13M18^T formed creamy-pink colonies on a marine agar plate. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indi-cated that strain K13M18^T was most closely related to Tabrizicola sediminis DRYC-M-16^T, sharing 97.62 % similarity with that strain. Strain K13M18^T belonged to the genus Tabrizicola, which formed a cluster with Tabrizicola aquatica RCRI19^T, Tabrizicola fusiformis SY72^T, Tabrizicola sediminis DRYC-M-16^T and Tabrizicola alkalilacus DJC^T in a phylogenetic tree based on the 16S rRNA gene sequences. Strain K13M18^T grown optimally in 0 % (w/v) NaCl, at pH 7 and 30 °C, in a marine broth medium. The predominant cellular fatty acids were C_{18: 1} ω7c and C_{18: 1} ω6c. The major respiratory isoprenoid quinone was ubiquinone Q-10. Polar lipids of strain K13M18^T contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglyc-erol, six unidentified aminophospholipids, one unidentified aminolipid and an unidentified lipid. Based on genome sequencing, the DNA G+C content of strain K13M18^T was 64.08 mol %, with an average nucleotide identity value, calculated by a comparative genomic analysis of strains K13M18^T and T. sediminis DRYC-M-16^T, of 74.82 %. Based on the phylogenetic, genotypic, and phenotypic information, strain K13M18^T is proposed to be a novel species of the genus Tabrizicola. The type strain is K13M18^T (=KCTC 62659^T=JCM 33230^T).