Taraxinic acid, a hydrolysate of sesquiterpene lactone glycoside from the Taraxacum coreanum NAKAI, induces the differentiation of human acute promyelocytic leukemia HL-60 cells

Jung Hye Choi, Kyung Min Shin, Na Young Kim, Jung Pyo Hong, Yong Sup Lee, Hyoung Ja Kim, Hee Juhn Park, Kyung Tae Lee

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The present work was performed to elucidate the active moiety of a sesquiterpene lactone, taraxinic acid-1′- O-β-D-glucopyranoside (1) from Taraxacum coreanum NAKAI on the cytotoxicity of various cancer cells. Based on enzymatic hydrolysis and MTT assay, the active moiety should be attributed to the aglycone taraxinic acid (1a), rather than the glycoside (1). Taraxinic acid exhibited potent antiproliferative activity against human leukemia- derived HL-60. In addition, this compound was found to be a potent inducer of HL-60 cell differentiation as assessed by a nitroblue tetrazolium reduction test, esterase activity assay, phagocytic activity assay, morphology change, and expression of CD14 and CD66b surface antigens. These results suggest that taraxinic acid induces the differentiation of human leukemia cells to monocyte/macrophage lineage. Moreover, the expression level of c- myc was down-regulated during taraxinic acid-dependent HL-60 cell differentiation, whereas p21CIP1 and p27KIP1 were up-regulated. Taken together, our results suggest that taraxinic acid may have potential as a therapeutic agent in human leukemia.

Original languageEnglish
Pages (from-to)1446-1450
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume25
Issue number11
DOIs
Publication statusPublished - Nov 2002

Keywords

  • Differentiation
  • HL-60 cell
  • Taraxinic acid
  • c-myc
  • p21
  • p27

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