The multi-targeted effects of chrysanthemum herb extract against Escherichia coli O157:H7

Ki Suk Kim, Dong Jin Lim, Hea Jung Yang, Eun Kyeong Choi, Min Hee Shin, Kwang Seok Ahn, Sang Hoon Jung, Jae Young Um, Hee Jae Jung, Jun Hee Lee, Seok Geun Lee, Sung Ki Jung, Hyeung Jin Jang

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The Chrysanthemum lavandulifolium extract, which includes chrysoeriol, sudachitin, and acacetin, has excellent antibiotic effects on Escherichia coli O157:H7 (E. coli O157). A notable point is that the antibiotic targets of the herb extract are similar to the targets of commonly used antibiotic drugs, including bacterial cell wall biosynthesis, bacterial protein synthesis, and bacterial DNA replication and repair. In addition, the herbal antibiotic inhibits the etiological factors that contribute to the pathogenic property. The herbal sample was extracted and fractionated and then inoculated through a disk diffusion method to confirm its antibiotic effect against E. coli O157. Total RNA was isolated from the affected bacterial cells, and its expression level was analyzed through a microarray analysis. To confirm the accuracy of the microarray data, a real-time PCR was performed. Three active compounds, chrysoeriol, sudachitin, and acacetin, were identified with a high-performance liquid chromatography-electrospray ionization/mass spectrometry chromatogram, and the disk diffusion study confirmed that chrysoeriol and sudachitin contribute to the antibiotic properties of the herb extract. The results demonstrate that the multi-target efficacy of the herbal sample may indicate the potential for the development of more effective and safer drugs that will act as substitutes for existing antibiotics.

Original languageEnglish
Pages (from-to)1398-1406
Number of pages9
JournalPhytotherapy Research
Volume27
Issue number9
DOIs
Publication statusPublished - Sept 2013

Keywords

  • Chrysanthemum lavandulifolium
  • Escherichia coli O157:H7
  • antibiotics
  • microarray

Fingerprint

Dive into the research topics of 'The multi-targeted effects of chrysanthemum herb extract against Escherichia coli O157:H7'. Together they form a unique fingerprint.

Cite this