Two leucines in the N-terminal MAPK-docking site of tomato SlMKK2 are critical for interaction with a downstream MAPK to elicit programmed cell death associated with plant immunity

Chang Sik Oh; Jeena Hwang; Min Seon Choi; Byoung Cheorl Kang; Gregory B. Martin

doi:10.1016/j.febslet.2013.03.033

Two leucines in the N-terminal MAPK-docking site of tomato SlMKK2 are critical for interaction with a downstream MAPK to elicit programmed cell death associated with plant immunity

Chang Sik Oh
, Jeena Hwang
, Min Seon Choi
, Byoung Cheorl Kang
, Gregory B. Martin

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Tomato MAPK kinase SlMKK2 is a key protein regulating immunity-associated programmed cell death (PCD) in plants. We examined the role of the N-terminal MAPK-docking site (or D-site) of SlMKK2 in PCD elicitation. In vivo assays revealed that SlMKK2 interacted with the downstream MAPK SlMPK3 independent of PCD elicitation and two conserved leucines in the D-site were required for both interaction with SlMPK3 and PCD elicitation. These results demonstrate that two leucines in the D-site of SlMKK2 play a critical role in regulation of signal transfer to the downstream MAPK by regulating their physical interaction.

Original language	English
Pages (from-to)	1460-1465
Number of pages	6
Journal	FEBS Letters
Volume	587
Issue number	10
DOIs	https://doi.org/10.1016/j.febslet.2013.03.033
Publication status	Published - 21 May 2013

Bibliographical note

Funding Information:
This work was supported by a Grant from the Kyung Hee University in 2011 ( KHU-20110454 ) and by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government ( NRF-2011-0012016 ) to C.S.O. This work was also supported by Grants from the Israel-USA Binational Agriculture Research and Development Fund ( IS-4159-08C and IS-4510-12C ) to G.B.M. and a Grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ009065012012 ), Rural Development Administration, Republic of Korea, to J.H. and B.C.K.

Keywords

Cell death
D-site
Disease resistance
MAPK

Access to Document

10.1016/j.febslet.2013.03.033

Cite this

@article{a09c65525aaa40fa86a110768aeb3a6c,

title = "Two leucines in the N-terminal MAPK-docking site of tomato SlMKK2 are critical for interaction with a downstream MAPK to elicit programmed cell death associated with plant immunity",

abstract = "Tomato MAPK kinase SlMKK2 is a key protein regulating immunity-associated programmed cell death (PCD) in plants. We examined the role of the N-terminal MAPK-docking site (or D-site) of SlMKK2 in PCD elicitation. In vivo assays revealed that SlMKK2 interacted with the downstream MAPK SlMPK3 independent of PCD elicitation and two conserved leucines in the D-site were required for both interaction with SlMPK3 and PCD elicitation. These results demonstrate that two leucines in the D-site of SlMKK2 play a critical role in regulation of signal transfer to the downstream MAPK by regulating their physical interaction.",

keywords = "Cell death, D-site, Disease resistance, MAPK",

author = "Oh, \{Chang Sik\} and Jeena Hwang and Choi, \{Min Seon\} and Kang, \{Byoung Cheorl\} and Martin, \{Gregory B.\}",

note = "Funding Information: This work was supported by a Grant from the Kyung Hee University in 2011 ( KHU-20110454 ) and by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government ( NRF-2011-0012016 ) to C.S.O. This work was also supported by Grants from the Israel-USA Binational Agriculture Research and Development Fund ( IS-4159-08C and IS-4510-12C ) to G.B.M. and a Grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ009065012012 ), Rural Development Administration, Republic of Korea, to J.H. and B.C.K.",

year = "2013",

month = may,

day = "21",

doi = "10.1016/j.febslet.2013.03.033",

language = "English",

volume = "587",

pages = "1460--1465",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "10",

}

TY - JOUR

T1 - Two leucines in the N-terminal MAPK-docking site of tomato SlMKK2 are critical for interaction with a downstream MAPK to elicit programmed cell death associated with plant immunity

AU - Oh, Chang Sik

AU - Hwang, Jeena

AU - Choi, Min Seon

AU - Kang, Byoung Cheorl

AU - Martin, Gregory B.

N1 - Funding Information: This work was supported by a Grant from the Kyung Hee University in 2011 ( KHU-20110454 ) and by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government ( NRF-2011-0012016 ) to C.S.O. This work was also supported by Grants from the Israel-USA Binational Agriculture Research and Development Fund ( IS-4159-08C and IS-4510-12C ) to G.B.M. and a Grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ009065012012 ), Rural Development Administration, Republic of Korea, to J.H. and B.C.K.

PY - 2013/5/21

Y1 - 2013/5/21

N2 - Tomato MAPK kinase SlMKK2 is a key protein regulating immunity-associated programmed cell death (PCD) in plants. We examined the role of the N-terminal MAPK-docking site (or D-site) of SlMKK2 in PCD elicitation. In vivo assays revealed that SlMKK2 interacted with the downstream MAPK SlMPK3 independent of PCD elicitation and two conserved leucines in the D-site were required for both interaction with SlMPK3 and PCD elicitation. These results demonstrate that two leucines in the D-site of SlMKK2 play a critical role in regulation of signal transfer to the downstream MAPK by regulating their physical interaction.

AB - Tomato MAPK kinase SlMKK2 is a key protein regulating immunity-associated programmed cell death (PCD) in plants. We examined the role of the N-terminal MAPK-docking site (or D-site) of SlMKK2 in PCD elicitation. In vivo assays revealed that SlMKK2 interacted with the downstream MAPK SlMPK3 independent of PCD elicitation and two conserved leucines in the D-site were required for both interaction with SlMPK3 and PCD elicitation. These results demonstrate that two leucines in the D-site of SlMKK2 play a critical role in regulation of signal transfer to the downstream MAPK by regulating their physical interaction.

KW - Cell death

KW - D-site

KW - Disease resistance

KW - MAPK

UR - https://www.scopus.com/pages/publications/84877761907

U2 - 10.1016/j.febslet.2013.03.033

DO - 10.1016/j.febslet.2013.03.033

M3 - Article

C2 - 23583714

AN - SCOPUS:84877761907

SN - 0014-5793

VL - 587

SP - 1460

EP - 1465

JO - FEBS Letters

JF - FEBS Letters

IS - 10

ER -

Two leucines in the N-terminal MAPK-docking site of tomato SlMKK2 are critical for interaction with a downstream MAPK to elicit programmed cell death associated with plant immunity

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this