Ultra-thin microchannel-type electrophoresis chip for TIRFM-based single-DNA observation in the femtomole concentration

Won Gu Lee; Keunchang Cho; Chanil Chung; Hoyoung Yun; Seok Chung; Seong Ho Kang; Dong Chul Han; Jun Keun Chang

doi:10.1016/j.cap.2006.01.026

Ultra-thin microchannel-type electrophoresis chip for TIRFM-based single-DNA observation in the femtomole concentration

Won Gu Lee, Keunchang Cho, Chanil Chung, Hoyoung Yun, Seok Chung, Seong Ho Kang, Dong Chul Han, Jun Keun Chang

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

In this article, ultra-thin microchannel-type electrophoresis chip is presented to enhance the detection sensitivity of total internal reflection fluorescence microscopy (TIRFM) for both prism- and lens-type methods simultaneously. Very small thickness of the microchip is in particular helpful to acquire the better images of biomolecules near the bottom wall in the prism-type TIRFM using an upright microscope. This microchip has salient features in that its whole thickness can lie between 200 μm and 250 μm, taken into account, relatively shorter working distance (WD, 1.5-1.7) of high numerical aperture (NA, 1.4-1.65) optics. The design specifications of the chip for a fine control of micro- and nano-fluidics in the femtomole concentration are described.

Original language	English
Pages (from-to)	e137-e140
Journal	Current Applied Physics
Volume	6
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/j.cap.2006.01.026
Publication status	Published - Aug 2006

Bibliographical note

Funding Information:
The authors specially thank Seok Jin Chung and Dae-Kwang Kim, at Chonbuk National University, Korea for valuable suggestions of DNA sample preparation. This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (02-PJ10-PG4-PT02-0042) and was partly supported by the SRC/ERC program of MOST/KOSEF (R11-2000-075-02001-0).

Keywords

Electrophoresis
Microchannel
Single-DNA observation
TIRFM

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10.1016/j.cap.2006.01.026

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@article{319d5ec0b6074e3e96acd80af1647e4a,

title = "Ultra-thin microchannel-type electrophoresis chip for TIRFM-based single-DNA observation in the femtomole concentration",

abstract = "In this article, ultra-thin microchannel-type electrophoresis chip is presented to enhance the detection sensitivity of total internal reflection fluorescence microscopy (TIRFM) for both prism- and lens-type methods simultaneously. Very small thickness of the microchip is in particular helpful to acquire the better images of biomolecules near the bottom wall in the prism-type TIRFM using an upright microscope. This microchip has salient features in that its whole thickness can lie between 200 μm and 250 μm, taken into account, relatively shorter working distance (WD, 1.5-1.7) of high numerical aperture (NA, 1.4-1.65) optics. The design specifications of the chip for a fine control of micro- and nano-fluidics in the femtomole concentration are described.",

keywords = "Electrophoresis, Microchannel, Single-DNA observation, TIRFM",

author = "Lee, {Won Gu} and Keunchang Cho and Chanil Chung and Hoyoung Yun and Seok Chung and Kang, {Seong Ho} and Han, {Dong Chul} and Chang, {Jun Keun}",

note = "Funding Information: The authors specially thank Seok Jin Chung and Dae-Kwang Kim, at Chonbuk National University, Korea for valuable suggestions of DNA sample preparation. This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (02-PJ10-PG4-PT02-0042) and was partly supported by the SRC/ERC program of MOST/KOSEF (R11-2000-075-02001-0).",

year = "2006",

month = aug,

doi = "10.1016/j.cap.2006.01.026",

language = "English",

volume = "6",

pages = "e137--e140",

journal = "Current Applied Physics",

issn = "1567-1739",

publisher = "Elsevier B.V.",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Ultra-thin microchannel-type electrophoresis chip for TIRFM-based single-DNA observation in the femtomole concentration

AU - Lee, Won Gu

AU - Cho, Keunchang

AU - Chung, Chanil

AU - Yun, Hoyoung

AU - Chung, Seok

AU - Kang, Seong Ho

AU - Han, Dong Chul

AU - Chang, Jun Keun

N1 - Funding Information: The authors specially thank Seok Jin Chung and Dae-Kwang Kim, at Chonbuk National University, Korea for valuable suggestions of DNA sample preparation. This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (02-PJ10-PG4-PT02-0042) and was partly supported by the SRC/ERC program of MOST/KOSEF (R11-2000-075-02001-0).

PY - 2006/8

Y1 - 2006/8

N2 - In this article, ultra-thin microchannel-type electrophoresis chip is presented to enhance the detection sensitivity of total internal reflection fluorescence microscopy (TIRFM) for both prism- and lens-type methods simultaneously. Very small thickness of the microchip is in particular helpful to acquire the better images of biomolecules near the bottom wall in the prism-type TIRFM using an upright microscope. This microchip has salient features in that its whole thickness can lie between 200 μm and 250 μm, taken into account, relatively shorter working distance (WD, 1.5-1.7) of high numerical aperture (NA, 1.4-1.65) optics. The design specifications of the chip for a fine control of micro- and nano-fluidics in the femtomole concentration are described.

AB - In this article, ultra-thin microchannel-type electrophoresis chip is presented to enhance the detection sensitivity of total internal reflection fluorescence microscopy (TIRFM) for both prism- and lens-type methods simultaneously. Very small thickness of the microchip is in particular helpful to acquire the better images of biomolecules near the bottom wall in the prism-type TIRFM using an upright microscope. This microchip has salient features in that its whole thickness can lie between 200 μm and 250 μm, taken into account, relatively shorter working distance (WD, 1.5-1.7) of high numerical aperture (NA, 1.4-1.65) optics. The design specifications of the chip for a fine control of micro- and nano-fluidics in the femtomole concentration are described.

KW - Electrophoresis

KW - Microchannel

KW - Single-DNA observation

KW - TIRFM

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DO - 10.1016/j.cap.2006.01.026

M3 - Article

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SN - 1567-1739

VL - 6

SP - e137-e140

JO - Current Applied Physics

JF - Current Applied Physics

IS - SUPPL. 1

ER -

Ultra-thin microchannel-type electrophoresis chip for TIRFM-based single-DNA observation in the femtomole concentration

Abstract

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Keywords

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