TY - GEN
T1 - Ultrastructural and mechanical effects of collagen cross-linking treatment on human corneo-scleral tissues
AU - Choi, S.
AU - Jung, G. B.
AU - Cheong, Y.
AU - Lee, S.
AU - Kim, J. K.
AU - Shin, J. H.
AU - Jin, K. H.
AU - Park, H. K.
PY - 2012
Y1 - 2012
N2 - The aim of this study was to quantitatively examine the immediate effects of a photooxidative collagen cross-linking (CxL) treatment with photosensitizer riboflavin (RF) and 370 nm UVA light in in vitro rabbit dermal collagen fibrils. These effects would be measured at the corneo-scleral (CS) stroma, 20 8 x 2 mm CS strips, based on clinical corneal CxL procedures on the morphological and mechanical response level using histology and AFM analyses. Five parameters including the density, area, adhesion force, and stiffness of CS tissues before and after RFUVA catalyzed collagen CxL treatment were investigated. The RFUVA catalyzed collagen CxL treatment led to an increase in the area and density of both corneal (>108%) and scleral (>118%) stromal collagens. Furthermore, RF-UVA catalyzed collagen CxL treatment led to an increased biomechanical response of CS; 125% for corneal stiffness and 108% for scleral stiffness, and 124% for corneal adhesion force and 122% for scleral adhesion force, p > 0.05. The collagen CxL treatment through RF-sensitized photoreaction may cause morphological and biomechanical property changes in the collagen fibril network of the cornea as well as the sclera, due to the stromal edema and interfibrillar spacing narrowing.
AB - The aim of this study was to quantitatively examine the immediate effects of a photooxidative collagen cross-linking (CxL) treatment with photosensitizer riboflavin (RF) and 370 nm UVA light in in vitro rabbit dermal collagen fibrils. These effects would be measured at the corneo-scleral (CS) stroma, 20 8 x 2 mm CS strips, based on clinical corneal CxL procedures on the morphological and mechanical response level using histology and AFM analyses. Five parameters including the density, area, adhesion force, and stiffness of CS tissues before and after RFUVA catalyzed collagen CxL treatment were investigated. The RFUVA catalyzed collagen CxL treatment led to an increase in the area and density of both corneal (>108%) and scleral (>118%) stromal collagens. Furthermore, RF-UVA catalyzed collagen CxL treatment led to an increased biomechanical response of CS; 125% for corneal stiffness and 108% for scleral stiffness, and 124% for corneal adhesion force and 122% for scleral adhesion force, p > 0.05. The collagen CxL treatment through RF-sensitized photoreaction may cause morphological and biomechanical property changes in the collagen fibril network of the cornea as well as the sclera, due to the stromal edema and interfibrillar spacing narrowing.
KW - Biomechinical property
KW - Corneo-scleral collagens
KW - Photooxidative collagen CxL treatment
KW - Riboflavin and UVA light
UR - http://www.scopus.com/inward/record.url?scp=84864976410&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84864976410
SN - 9781466562769
T3 - Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
SP - 250
EP - 253
BT - Technical Proceedings of the 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
T2 - Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy - 2012 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2012
Y2 - 18 June 2012 through 21 June 2012
ER -