Ursolic acid downregulates thymic stromal lymphopoietin through the blockade of intracellular calcium/caspase-1/NF-κB signaling cascade in HMC-1 cells

Phil Dong Moon, Na Ra Han, Jin Soo Lee, Hyung Min Kim, Hyun Ja Jeong

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Thymic stromal lymphopoietin (TSLP) plays an important role in allergic disorders, including atopic dermatitis and asthma. Ursolic acid (UA) has various pharmacological properties, such as antioxidant, anti-inflammatory and anticancer. However, the effect of UA on TSLP regulation has not been fully elucidated. The aim of the present study was to analyze how UA regulates the production of TSLP in the human mast cell line HMC- 1. Enzyme-linked immunosorbent assay, quantitative polymerase chain reaction analysis, western blotting, caspase-1 assay and fluorescent measurements of intracellular calcium levels were conducted to analyze the regulatory effects of UA. The results revealed that UA inhibited TSLP production and mRNA expression. In addition, UA reduced the activation of nuclear factor-κB and degradation of IκBα. Caspase-1 activity was increased by exposure to phorbol myristate acetate plus calcium ionophore, whereas it was reduced by UA. Finally, UA treatment prevented an increase in intracellular calcium levels. These results indicated that UA may be a useful agent for the treatment and/or prevention of atopic and inflammatory diseases, and its effects are likely mediated by TSLP downregulation.

Original languageEnglish
Pages (from-to)2252-2258
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume43
Issue number5
DOIs
Publication statusPublished - May 2019

Keywords

  • Caspase-1
  • Intracellular calcium
  • Nuclear factor-κB
  • Thymic stromal lymphopoietin
  • Ursolic acid

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