Use of physcion to improve atopic dermatitis-like skin lesions through blocking of thymic stromal lymphopoietin

Phil Dong Moon, Na Ra Han, Jin Soo Lee, Sungwei Hong, Min Sun Yoo, Hyeong Jin Kim, Ji Hyeon Kim, Soonsik Kang, Hyun Woo Jee, Hyung Min Kim, Hyun Ja Jeong

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Physcion is well known for the treatment of carcinoma. However, the therapeutic effect of physcion on atopic dermatitis (AD) through the inhibition of thymic stromal lymphopoietin (TSLP) level remains largely unknown. In this study, we investigated the anti-AD effect of physcion using HMC-1 cells, splenocytes, and a murine model. Treatment with physcion decreased production and mRNA expression levels of TSLP, IL-6, TNF-α, and IL-1β in activated HMC-1 cells. Physcion reduced the expression levels of RIP2/caspase-1 and phospho (p)ERK/pJNK/pp38 in activated HMC-1 cells. Physcion suppressed the expression levels of pIKKβ/NF-κB/pIkBα in activated HMC-1 cells. Moreover, physcion attenuated the production levels of TSLP, IL-4, IL-6, TNF-α, and IFN-γ from activated splenocytes. Oral administration of physcion improved the severity of 2,4-dinitrochlorobenzene-induced AD-like lesional skin through reducing infiltration of inflammatory cells and mast cells, and the protein and mRNA levels of TSLP, IL-4, and IL-6 in the lesional skin tissues. Physcion attenuated histamine, IgE, TSLP, IL-4, IL-6, and TNF-α levels in serum. In addition, physcion inhibited caspase-1 activation in the lesional skin tissues. These findings indicate that physcion could ameliorate AD-like skin lesions by inhibiting TSLP levels via caspase-1/MAPKs/NF-kB signalings, which would provide experimental evidence of the therapeutic potential of physcion for AD.

Original languageEnglish
Article number1484
JournalMolecules
Volume24
Issue number8
DOIs
Publication statusPublished - 15 Apr 2019

Keywords

  • Atopic dermatitis
  • Caspase-1
  • Mast cells
  • Physcion
  • Thymic stromal lymphopoietin

Fingerprint

Dive into the research topics of 'Use of physcion to improve atopic dermatitis-like skin lesions through blocking of thymic stromal lymphopoietin'. Together they form a unique fingerprint.

Cite this