TY - JOUR
T1 - Water extract of processed Hydrangea macrophylla (Thunb.) Ser. leaf attenuates the expression of pro-inflammatory mediators by suppressing Akt-mediated NF-κB activation
AU - Dilshara, Matharage Gayani
AU - Jayasooriya, Rajapaksha Gendara Prasad Tharanga
AU - Lee, Seungheon
AU - Jeong, Joon Bum
AU - Seo, Yong Taek
AU - Choi, Yung Hyun
AU - Jeong, Jin Woo
AU - Jang, Young Pyo
AU - Jeong, Yong Kee
AU - Kim, Gi Young
N1 - Funding Information:
This research was supported by the Technology Development Program for Agriculture and Forestry ( 610003-03-1-SB110 ), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
PY - 2013/3
Y1 - 2013/3
N2 - Although Hydrangea macrophylla is native to Northeast Asia and widely cultivated in many parts of the world, no studies on its anti-inflammatory effects have been reported. In this study, we evaluated the anti-inflammatory effect of a water extract of processed H. macrophylla leaf (WH) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. WH inhibited the expression of LPS-stimulated pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor-α (TNF-α), as well as their regulatory genes inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α without any accompanying cytotoxicity. Moreover, WH significantly suppressed the LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB), as well as the nuclear translocation of the NF-κB subunits, p65 and p50 by suppressing of IκBα phosphorylation and degradation. WH also increased Akt dephosphorylation, leading to the suppression of the DNA-binding activity of NF-κB in LPS-stimulated RAW264.7 macrophage cells. Our results indicate that WH downregulates the expression of pro-inflammatory mediators such as NO, PGE2, and TNF-α by suppressing the Akt-mediated NF-κB activity in LPS-stimulated RAW264.7 macrophage cells.
AB - Although Hydrangea macrophylla is native to Northeast Asia and widely cultivated in many parts of the world, no studies on its anti-inflammatory effects have been reported. In this study, we evaluated the anti-inflammatory effect of a water extract of processed H. macrophylla leaf (WH) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. WH inhibited the expression of LPS-stimulated pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor-α (TNF-α), as well as their regulatory genes inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α without any accompanying cytotoxicity. Moreover, WH significantly suppressed the LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB), as well as the nuclear translocation of the NF-κB subunits, p65 and p50 by suppressing of IκBα phosphorylation and degradation. WH also increased Akt dephosphorylation, leading to the suppression of the DNA-binding activity of NF-κB in LPS-stimulated RAW264.7 macrophage cells. Our results indicate that WH downregulates the expression of pro-inflammatory mediators such as NO, PGE2, and TNF-α by suppressing the Akt-mediated NF-κB activity in LPS-stimulated RAW264.7 macrophage cells.
KW - Hydrangea macrophylla
KW - Nitric oxide
KW - Nuclear factor-κB
KW - Prostaglandin E
KW - Tumor necrosis factor-α
UR - http://www.scopus.com/inward/record.url?scp=84873244692&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2012.12.012
DO - 10.1016/j.etap.2012.12.012
M3 - Article
C2 - 23376181
AN - SCOPUS:84873244692
SN - 1382-6689
VL - 35
SP - 311
EP - 319
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 2
ER -