Weissella diestrammenae sp. nov., isolated from the gut of a camel cricket (Diestrammena coreana)

A novel, Gram-stain-positive, non-motile, facultatively anaerobic, rod- or coccoid-shaped bacterium, designated strain ORY33^T, was isolated from the gut of a camel cricket (Diestrammena coreana). The 16S rRNA gene sequence analysis showed that strain ORY33^T belonged to the genus Weissella, with highest sequence similarity to Weissella koreensis S-5623^T (97.7%). The strain grew optimally at 30 °C and pH 7 in the presence of 0 % (w/v) NaCl. Catalase and oxidase activities were negative. The genomic DNA G+C content of strain ORY33^T was 45.1 mol%. DNA-DNA hybridization values between strain ORY33^T and closely related members of the genus Weissella were less than 27 %. The major fatty acids of strain ORY33^T were C_18:1ω9c, C_16:0 and C_14:0. Based on these phenotypic, phylogenetic and genotypic analyses, strain ORY33^T represents a novel species belonging to the genus Weissella, for which the name Weissella diestrammenae sp. nov. is proposed. The type strain is ORY33^T (5KACC 16890^T5JCM 18559^T).