Wohlfahrtiimonas larvae sp. Nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)

Jae Kook Lee; Youn Yeop Lee; Kwan Ho Park; Jeonggu Sim; Youngcheol Choi; Sung Jae Lee

doi:10.1007/s10482-013-0048-5

Wohlfahrtiimonas larvae sp. Nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)

Jae Kook Lee
, Youn Yeop Lee
, Kwan Ho Park
, Jeonggu Sim
, Youngcheol Choi
, Sung Jae Lee

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006^T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006^T showed 96.4 % similarity to that of Wohlfahrtiimonas chitiniclastica S5^T. Strain KBL006^T grew optimally at 30 C, at pH 8.0 and in the presence of 1-2 % (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C_18:1 ω7c, C_14:0, and C_16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol%. Based on these polyphasic data, strain KBL006^T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. Nov. is proposed. The type strain is KBL006^T (= KACC 16839^T = JCM 18424^T).

Original language	English
Pages (from-to)	15-21
Number of pages	7
Journal	Antonie van Leeuwenhoek
Volume	105
Issue number	1
DOIs	https://doi.org/10.1007/s10482-013-0048-5
Publication status	Published - Jan 2014

Bibliographical note

Funding Information:
Acknowledgments We thank Drs. Jin-Woo Bae and Seong Woon Roh for experimental advices. The G ? C content analysis was done at Jeju center, Korean Basic Sciences Institute. This work was supported by the Rural Development Administration in Korea (PJ006893). JK Lee thanks to graduate student scholarship of Lotte foundation. SJ Lee thanks to support by NRF 2010-0010779.

Keywords

Hermetia illucens
Larval gut microorganism
Polyphasic taxonomy
Wohlfahrtiimonas larvae

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10.1007/s10482-013-0048-5

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title = "Wohlfahrtiimonas larvae sp. Nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)",

abstract = "A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006T showed 96.4 \% similarity to that of Wohlfahrtiimonas chitiniclastica S5T. Strain KBL006T grew optimally at 30 C, at pH 8.0 and in the presence of 1-2 \% (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol\%. Based on these polyphasic data, strain KBL006T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. Nov. is proposed. The type strain is KBL006T (= KACC 16839T = JCM 18424T).",

keywords = "Hermetia illucens, Larval gut microorganism, Polyphasic taxonomy, Wohlfahrtiimonas larvae",

author = "Lee, \{Jae Kook\} and Lee, \{Youn Yeop\} and Park, \{Kwan Ho\} and Jeonggu Sim and Youngcheol Choi and Lee, \{Sung Jae\}",

note = "Funding Information: Acknowledgments We thank Drs. Jin-Woo Bae and Seong Woon Roh for experimental advices. The G ? C content analysis was done at Jeju center, Korean Basic Sciences Institute. This work was supported by the Rural Development Administration in Korea (PJ006893). JK Lee thanks to graduate student scholarship of Lotte foundation. SJ Lee thanks to support by NRF 2010-0010779.",

year = "2014",

month = jan,

doi = "10.1007/s10482-013-0048-5",

language = "English",

volume = "105",

pages = "15--21",

journal = "Antonie van Leeuwenhoek",

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T1 - Wohlfahrtiimonas larvae sp. Nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)

AU - Lee, Jae Kook

AU - Lee, Youn Yeop

AU - Park, Kwan Ho

AU - Sim, Jeonggu

AU - Choi, Youngcheol

AU - Lee, Sung Jae

N1 - Funding Information: Acknowledgments We thank Drs. Jin-Woo Bae and Seong Woon Roh for experimental advices. The G ? C content analysis was done at Jeju center, Korean Basic Sciences Institute. This work was supported by the Rural Development Administration in Korea (PJ006893). JK Lee thanks to graduate student scholarship of Lotte foundation. SJ Lee thanks to support by NRF 2010-0010779.

PY - 2014/1

Y1 - 2014/1

N2 - A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006T showed 96.4 % similarity to that of Wohlfahrtiimonas chitiniclastica S5T. Strain KBL006T grew optimally at 30 C, at pH 8.0 and in the presence of 1-2 % (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol%. Based on these polyphasic data, strain KBL006T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. Nov. is proposed. The type strain is KBL006T (= KACC 16839T = JCM 18424T).

AB - A novel, Gram-negative, facultative anaerobic, motile and short rod-shaped bacterium, strain KBL006T was isolated from the larval gut of Hermetia illucens, Black soldier fly. The 16S rRNA gene sequence of strain KBL006T showed 96.4 % similarity to that of Wohlfahrtiimonas chitiniclastica S5T. Strain KBL006T grew optimally at 30 C, at pH 8.0 and in the presence of 1-2 % (w/v) NaCl. Oxidase activity and catalase activity were positive. The major fatty acids were C18:1 ω7c, C14:0, and C16:0. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, and two phospholipids. The G+C content of the genomic DNA was 45.2 mol%. Based on these polyphasic data, strain KBL006T is considered to represent a novel species in the genus Wohlfahrtiimonas, for which the name Wohlfahrtiimonas larvae sp. Nov. is proposed. The type strain is KBL006T (= KACC 16839T = JCM 18424T).

KW - Hermetia illucens

KW - Larval gut microorganism

KW - Polyphasic taxonomy

KW - Wohlfahrtiimonas larvae

UR - https://www.scopus.com/pages/publications/84892735444

U2 - 10.1007/s10482-013-0048-5

DO - 10.1007/s10482-013-0048-5

M3 - Article

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AN - SCOPUS:84892735444

SN - 0003-6072

VL - 105

SP - 15

EP - 21

JO - Antonie van Leeuwenhoek

JF - Antonie van Leeuwenhoek

IS - 1

ER -

Wohlfahrtiimonas larvae sp. Nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)

Abstract

Bibliographical note

Keywords

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