X-ray texture analysis indicates downward spinning of chitin microfibrils in tubeworm tube

Yu Ogawa; Kayoko Kobayashi; Satoshi Kimura; Yoshiharu Nishiyama; Masahisa Wada; Shigenori Kuga

doi:10.1016/j.jsb.2013.10.008

X-ray texture analysis indicates downward spinning of chitin microfibrils in tubeworm tube

Yu Ogawa
, Kayoko Kobayashi
, Satoshi Kimura
, Yoshiharu Nishiyama
, Masahisa Wada
, Shigenori Kuga

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The direction of β-chitin deposition in the tube of tubeworm Lamellibrachia satsuma was investigated by texture analysis using X-ray diffraction. The β-chitin crystallite in the tube has planar orientation with the (1. 1. 0) plane perpendicular to the surface, and the c-axis is aligned parallel to the tube. The monoclinic unit cell of β-chitin allowed determination of the sense of c-axis from the orientation of (0. 1. 0) and (1. 0. 0) planes. This means that the reducing end of β-chitin is pointing up in the tube. This orientation can be ascribed to possible secretion mechanisms of the β-chitin microfibrils, i.e. the chitin-synthesizing enzyme complex travels unidirectionally from top to bottom when the worm body contracts in the tube.

Original language	English
Pages (from-to)	212-216
Number of pages	5
Journal	Journal of Structural Biology
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/j.jsb.2013.10.008
Publication status	Published - Nov 2013

Bibliographical note

Funding Information:
We thank the Japan Agency for Marine-Earth Science and Technology (JAMSTEC) for collecting samples of L. satsuma using a remotely operated vehicle, Hyper-Dolphin. This study was supported in part by Grant-in-Aids for JSPS research fellow ( 23-2362 to Y.O., 23-2511 to K.K.).

Keywords

Chitin
Lamellibrachia satsuma
Microfibril
Tubeworm
X-ray diffraction

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10.1016/j.jsb.2013.10.008

Cite this

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title = "X-ray texture analysis indicates downward spinning of chitin microfibrils in tubeworm tube",

abstract = "The direction of β-chitin deposition in the tube of tubeworm Lamellibrachia satsuma was investigated by texture analysis using X-ray diffraction. The β-chitin crystallite in the tube has planar orientation with the (1. 1. 0) plane perpendicular to the surface, and the c-axis is aligned parallel to the tube. The monoclinic unit cell of β-chitin allowed determination of the sense of c-axis from the orientation of (0. 1. 0) and (1. 0. 0) planes. This means that the reducing end of β-chitin is pointing up in the tube. This orientation can be ascribed to possible secretion mechanisms of the β-chitin microfibrils, i.e. the chitin-synthesizing enzyme complex travels unidirectionally from top to bottom when the worm body contracts in the tube.",

keywords = "Chitin, Lamellibrachia satsuma, Microfibril, Tubeworm, X-ray diffraction",

author = "Yu Ogawa and Kayoko Kobayashi and Satoshi Kimura and Yoshiharu Nishiyama and Masahisa Wada and Shigenori Kuga",

note = "Funding Information: We thank the Japan Agency for Marine-Earth Science and Technology (JAMSTEC) for collecting samples of L. satsuma using a remotely operated vehicle, Hyper-Dolphin. This study was supported in part by Grant-in-Aids for JSPS research fellow ( 23-2362 to Y.O., 23-2511 to K.K.).",

year = "2013",

month = nov,

doi = "10.1016/j.jsb.2013.10.008",

language = "English",

volume = "184",

pages = "212--216",

journal = "Journal of Structural Biology",

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T1 - X-ray texture analysis indicates downward spinning of chitin microfibrils in tubeworm tube

AU - Ogawa, Yu

AU - Kobayashi, Kayoko

AU - Kimura, Satoshi

AU - Nishiyama, Yoshiharu

AU - Wada, Masahisa

AU - Kuga, Shigenori

N1 - Funding Information: We thank the Japan Agency for Marine-Earth Science and Technology (JAMSTEC) for collecting samples of L. satsuma using a remotely operated vehicle, Hyper-Dolphin. This study was supported in part by Grant-in-Aids for JSPS research fellow ( 23-2362 to Y.O., 23-2511 to K.K.).

PY - 2013/11

Y1 - 2013/11

N2 - The direction of β-chitin deposition in the tube of tubeworm Lamellibrachia satsuma was investigated by texture analysis using X-ray diffraction. The β-chitin crystallite in the tube has planar orientation with the (1. 1. 0) plane perpendicular to the surface, and the c-axis is aligned parallel to the tube. The monoclinic unit cell of β-chitin allowed determination of the sense of c-axis from the orientation of (0. 1. 0) and (1. 0. 0) planes. This means that the reducing end of β-chitin is pointing up in the tube. This orientation can be ascribed to possible secretion mechanisms of the β-chitin microfibrils, i.e. the chitin-synthesizing enzyme complex travels unidirectionally from top to bottom when the worm body contracts in the tube.

AB - The direction of β-chitin deposition in the tube of tubeworm Lamellibrachia satsuma was investigated by texture analysis using X-ray diffraction. The β-chitin crystallite in the tube has planar orientation with the (1. 1. 0) plane perpendicular to the surface, and the c-axis is aligned parallel to the tube. The monoclinic unit cell of β-chitin allowed determination of the sense of c-axis from the orientation of (0. 1. 0) and (1. 0. 0) planes. This means that the reducing end of β-chitin is pointing up in the tube. This orientation can be ascribed to possible secretion mechanisms of the β-chitin microfibrils, i.e. the chitin-synthesizing enzyme complex travels unidirectionally from top to bottom when the worm body contracts in the tube.

KW - Chitin

KW - Lamellibrachia satsuma

KW - Microfibril

KW - Tubeworm

KW - X-ray diffraction

UR - https://www.scopus.com/pages/publications/84887227504

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DO - 10.1016/j.jsb.2013.10.008

M3 - Article

C2 - 24145302

AN - SCOPUS:84887227504

SN - 1047-8477

VL - 184

SP - 212

EP - 216

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 2

ER -

X-ray texture analysis indicates downward spinning of chitin microfibrils in tubeworm tube

Abstract

Bibliographical note

Keywords

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