Abstract
This paper reports the development of a high-throughput ZnO nanowire incorporated microdevice for microalgal cell transformation, in which the cellular attachment and detachment from the nanowires can be modulated through the operation of a pneumatic PDMS microvalve. We demonstrated superior nanowire-mediated gene transfer by tuning the pneumatic microvalve, and the target gene was inserted to microalgal cell with a high viability and gene integrity, which can provide an advanced genetic engineering tool.
| Original language | English |
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| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 126-128 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Bibliographical note
Publisher Copyright:© 14CBMS.
Keywords
- Microalgal cell
- Microfluidics
- Pneumatic pressure
- Transformation
- ZnO nanowire